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Erythromycin ELISA kit

1. Principle Erythromycin ELISA kit is a competitive enzyme immunoassay for the quantitative analysis of Erythromycin in milk, honey and meat. The coupling antigens are coated on the micro-well stripes. The Erythromycin in the sample and the coupling antigens coated on the micro-well stripes compete for the anti-Erythromycin antibodies. After the addition of the enzyme conjugate, the TMB su...

1. Principle
Erythromycin ELISA kit is a competitive enzyme immunoassay for the quantitative analysis of Erythromycin in milk, honey and meat.
The coupling antigens are coated on the micro-well stripes. The Erythromycin in the sample and the coupling antigens coated on the micro-well stripes compete for the anti-Erythromycin antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration.
The optical density (OD) value of the sample has a negative correlation with the Erythromycin in it. The measurement is made photometrically at 450 nm. The absorption is inversely proportional to the Erythromycin concentration in the sample.

2. Technical specification:
Sensitivity: 0.1 ppb Detect time: Approx.75 min

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