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Tetracyclines(TCs) ELISA Test Kit

1. Principle Tetracyclines ELISA kit is a competitive enzyme immunoassay for the quantitative analysis of tetracycline, rolitetracycline, chlortetracycline demeclocycline,oxytetracycline and doxycycline in milk, honey and meat.The coupling antigens are coated on the micro-well stripes. The Tetracyclines in the sample and the coupling antigens coated on the micro-well stripes compete for ...

1. Principle
Tetracyclines ELISA kit is a competitive enzyme immunoassay for the quantitative analysis of tetracycline, rolitetracycline, chlortetracycline demeclocycline,oxytetracycline and doxycycline in milk, honey and meat.The coupling antigens are coated on the micro-well stripes. The Tetracyclines in the sample and the coupling antigens coated on the micro-well stripes compete for the anti-Tetracyclines antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Tetracyclines in it. The measurement is made photometrically at 450 nm. The absorption is inversely proportional to the tetracyclines concentration in the sample.

2. Technical specification:
Sensitivity: 0.05 ppb Detect time: Approx.75 min

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