Ethoxyquin ELISA kit

Introduction
Ethoxyquin is assigned a toxicity rating of 3 or "moderately toxic", indicating the probable oral lethal human dose is 0.5-5 g/kg, 3- to 33-times the maximum allowed in pet foods. This toxicity rating is slightly greater than ratings for tetracycline and penicillin, lower than for aspirin and caffeine. Susceptibility of laboratory animals to anti-oxidant toxicity increases with the nutritional stress of variable dietary constituents. Increased dietary fat, for example, increases susceptibility to toxicity of ethoxyquin fed to chickens and BHT (as well as DDT) fed to rats. The response in chickens to increased dietary fat appeared to be due to the resultant lowered protein. Chickens fed17vs. 23% protein showed increased susceptibility to ethoxyquin toxicity. Ethoxquin levels fed to chickens were, however, almost 17 times the maximum allowable level for pet foods.

Principle
This assay employs the indirect competitive inhibition enzyme immunoassay technique. A Ethoxyquin conjugate has been pre-coated onto a microplate. Add HRP-anti-Ethoxyquin antibody and Ethoxyquin (Standards or samples) to the well. A competitive inhibition reaction is launched between pre-coated Ethoxyquin and Ethoxyquin (Standards or samples) with the HRP-anti-Ethoxyquin antibody. The substrate solutions are added to the wells, respectively. And the color develops in opposite to the amount of Ethoxyquin bound in the initial step. The color development is stopped and the intensity of the color is measured.

Specification
96 tests/kit